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A two-week gap separated every two courses, during which tamoxifen citrate (40 mg per day) and clomiphene citrate (10 mg per day) were taken to control serum testosterone levelswith a high-performance liquid chromatography system (Eclipse, USA). At the outset, serum testosterone levels were measured in a separate laboratory and then again after the second week of the treatment (two treatment trials). We tested the results with an independent control group in which a placebo was given orally at the same weekly doses. The results of the tests were compared by Student's t test. We also examined whether tamoxifen or clomiphene inhibited the aromatase activity in a two-week-old male rat by using a two-day-old, male rat with aromatase insensitivity at the end of the first week of treatment ( ). The treatment trials did not show a significant change in estradiol levels despite a significant increase in free testosterone levels ( ). There were no differences in the serum estradiol concentrations (5.3 ± 0.5 IU/l versus 6.8 ± 0.6 IU/l; P > 0.10). Therefore, the aromatase activity of the test hormone was unaffected by the treatment. The only exception was in serum free testosterone concentrations following treatment with ginseng root. In rats that received ginseng root (150 mg/kg per day) at the beginning of the treatment trial (2) and the last, dose-dependent reduction of estradiol levels was noticed and reached asymptomatic levels by day 9. This was not observed in the control rats treated with tamoxifen or clomiphene ( ). However, in these rats, an initial increase in estradiol levels in the second week of treatment was followed by a decrease in estradiol levels in the last week of treatment. The treatment with ginseng root at the start of the treatment trial prevented the initial increase in estradiol levels observed in these rats, and in contrast to the control rats, a significant reduction in estradiol concentration was observed in a dose-dependent manner; however, the decrease in estradiol concentration reached asymptomatic levels by day 9. Thus, by the second week of the treatment trial in the control rats, estradiol levels had decreased by 40% compared with the control rats. In contrast, the ginseng treatment reduced the estradiol levels by 80% compared with the control rats. The reduction of estradiol with or without the ginseng treatment appeared to be dose dependent; however, this was not confirmed for a large number of different drugs. Taken together, the Related Article: